Rybenkov V. The flatness of mica treated with PL, as described in Materials and Methods, was the same as that of untreated mica, independently of the amount of PL, as verified by measuring roughness values using the NanoScope IIIa software on images recorded at constant scanning parameters data not shown. This difference is explained by enhanced DNA—surface interactions at low salt concentrations, where the negative charges of the DNA are less shielded.
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